The loss of signal, which happened at 11:19 GMT (12:19 BST; 13:19 CEST), was greeted by muted cheers and handshakes - not so surprising given the bittersweet nature of the occasion.
Some of the scientists watching on here in Darmstadt have spent the better part of 30 years on this project.
"People are very sad today but I think they really understand how proud we are and how proud they should be that we've pulled this mission off," said Esa's senior science advisor, Mark McCaughrean.
Throughout Friday morning, the instrument teams had followed every twist and turn as the probe aimed for a touchdown spot on the head of the 4km-wide, duck-shaped comet.
The researchers had wanted the descending probe to get a look inside one of the many pits that pockmark the surface.
These sinkholes are often the places where 67P ejects gas and dust into space. But they also afford an opportunity to look at the object's interior, to see the lumpy ice blocks that may have come together to build the comet billions of years ago.
Some of the images that came back were acquired just seconds before the collision. These pictures will have resolutions that can be measured in millimetres. "They're super-duper," enthused Holger Sierks, the head of the Osiris camera team. "I've got goosebumbs just thinking about all this," he told BBC News.
Comet 67P/Churyumov-Gerasimenko is currently heading away from the Sun, limiting the solar energy available to Rosetta to operate its systems.
Rather than put the probe into hibernation or simply let it slowly fade into inactivity, the mission team determined that the venture should try to go out with a bang.
European Space Agency project scientist Matt Taylor said that even if Rosetta was sent to sleep with the intention of waking it up again when 67P next visited the brighter environs of the inner Solar System - there was no guarantee the technology would still be working properly.
"It's like one of those 60s rock bands; we don't want to have a rubbish comeback tour. We'd rather go out now in true rock'n'roll style," he said just before landing.
Because Rosetta was not designed to touchdown, some of its structures very likely broke on contact with the comet. Controllers left no room for doubt in any case by pre-loading a software sequence that would jump the computers into a shutdown when the probe felt the impact jolt.
Rosetta arrived at 67P/Churyumov-Gerasimenko - to give the comet its full name - in August 2014, after a 10-year journey from Earth.
In the time it has lived alongside the mountainous object, it has unlocked the secrets about its behaviour, its structure and chemistry.
Rosetta even dropped a small robot called Philae on to the surface in November 2014 to gather additional information - a historic first in space exploration.
The European Space Agency says the mission has been an outstanding success and will transform our understanding of the huge icy dirt-balls that wander among the planets.
The American scientist Alan Stern, whose Alice instrument has made far-ultraviolet observations of the comet to study composition and activity, said all the science teams involved still had much work to do: "We've got 70,000 spectra; we've barely scratched the surface in terms of looking at the data."